Enhanced Transcriptional Activation in Developing Mouse Photoreceptors.

ABSTRACT:

Purpose

Retinal development in the mouse continues past birth and provides a widely used model system in which photoreceptor formation can be observed and manipulated. This experimental paradigm provides opportunities for both gain-of-function and loss-of-function studies, which can be accomplished through in vivo or ex vivo plasmid delivery and electroporation. However, the cis-regulatory elements used to implement this approach have not been fully evaluated or optimized for the unique transcriptional environment of photoreceptors.

Methods

Here we investigate whether the use of a photoreceptor cis-regulatory element from the Crx gene in combination with broadly active promoter elements can increase the targeting of developing photoreceptors in the mouse. This study characte

SUBMITTER: Patierno BM 

PROVIDER: S-EPMC11760266 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature