Development of a replication competent murine norovirus reporter system.

ABSTRACT: Caliciviruses are significant agricultural and human pathogens that are poorly understood due to the dearth of molecular tools, including reporter systems. We report the development of a stable, faithful, and robust luciferase-based reporter system for a model calicivirus, murine norovirus (MNoV). Genetic insertion of a HiBiT tag, an 11 amino acid fragment of nanolucifersase, at the junction of the nonstructural proteins NS4 and NS5 yields infectious virus. The resultant MNoV-HiBiT produces robust signal that is detected early in infection and occurs only in cells susceptible to MNoV infection. As proof of principle, we used this tool to characterize two unappreciated host directed anti-MNoV compounds. The use of the MNoV-HiBiT virus enables new mechanistic studies by a rapid and quantitat

SUBMITTER: Olson MC 

PROVIDER: S-EPMC11760427 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature