Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria.

ABSTRACT: Background/Objectives: Plasmid-mediated resistance is a significant mechanism that contributes to the gradual decrease in the efficacy of antibiotics from various classes, including carbapenems. The aim of this study is to investigate the frequency of transfer of carbapenemase-encoding plasmids from K. pneumoniae to E. coli and P. aeruginosa. Methods: Matings were performed on agar with subsequent isolation of transconjugant, recipient, and donor colonies. The frequency of conjugation (CF) and minimum inhibitory concentrations (MICs) of meropenem were determined for the PCR-confirmed transconjugants. A pharmacodynamic study was conducted using a hollow-fiber infection model on E. coli transconjugant in order to evaluate its viability in the presenc

SUBMITTER: Savelieva JR 

PROVIDER: S-EPMC11760439 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature