NF-κB-Inducing Kinase Is Essential for Effective c-Rel Transactivation and Binding to the <i>Il12b</i> Promoter in Macrophages.

ABSTRACT: This study investigates the role of NIK in activating specific inflammatory genes in macrophages, focusing on the effect of a mutation in NIK found in alymphoplasia (aly/aly) mice. Mouse peritoneal macrophages from aly/aly mice showed a severe defect in the production of some pro-inflammatory cytokines, such as IL-12. This effect seemed to take place at the transcriptional level, as shown by the reduced transcription of Il12b and Il12a in aly/aly macrophages after exposure to the TLR4 agonist LPS. Immunoprecipitation studies showed that the binding of NIK to c-Rel was not efficient in RAW 264.7 cells over-expressing the aly/aly mutation. In addition, the shuttling of c-Rel to the nucleus was shown to be impaired in al

SUBMITTER: Cuesta N 

PROVIDER: S-EPMC11760456 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature