Developing affordable and efficient heating devices for enhanced live cell imaging in confocal microscopy.

ABSTRACT: Temperature control is crucial for live cell imaging, particularly in studies involving plant responses to high ambient temperatures and thermal stress. This study presents the design, development, and testing of two cost-effective heating devices tailored for confocal microscopy applications: an aluminum heat plate and a wireless mini-heater. The aluminum heat plate, engineered to integrate seamlessly with the standard 160 mm × 110 mm microscope stage, supports temperatures up to 36°C, suitable for studies in the range of non-stressful warm temperatures (e.g., 25-27°C for Arabidopsis thaliana) and moderate heat stress (e.g., 30-36°C for A. thaliana). We also developed a wireless mini-heater that offers rapid, precise heating directly at the sample slide, with a temperature i

SUBMITTER: Bajracharya A 

PROVIDER: S-EPMC11760603 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature