Crb3 and NF2: A dynamic duo that controls assembly of the apical junctions and barrier function via Rho/ROCK signaling.

ABSTRACT: The gastrointestinal epithelium serves as a critical barrier separating intestinal lumen contents from the underlying tissue environment. Structure and function of the apical junctional complex (AJC), comprising tight and adherens junctions, are essential for establishing and maintaining a polarized and functional epithelial barrier. In this study, we investigated mechanisms by which an apical polarity protein Crumbs homolog 3 (CRB3) regulates AJC assembly and barrier function in primary murine intestinal epithelial cells. Using primary colonic epithelial cells (colonoids) derived from inducible and conditional Crb3 knockdown (Crb3^ERΔIEC) and control mice (Crb3^fl/fl), we demonstrate that Crb3 loss leads to compromised epithelial barrier function that was associated wi

SUBMITTER: Fan S 

PROVIDER: S-EPMC11760739 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature