Q - score as a reliability measure for protein, nucleic acid, and small molecule atomic coordinate models derived from 3DEM density maps.

ABSTRACT: Atomic coordinate models are important in the interpretation of 3D maps produced with cryoEM and sub-tomogram averaging in cryoET, or more generically, 3D electron microscopy (3DEM). In addition to visual inspection of such maps and models, quantitative metrics convey the reliability of the atomic coordinates, in particular how well the model is supported by the experimentally determined 3DEM map. A recently introduced metric, Q-score , was shown to correlate well with the reported resolution of the map for well-fitted models. Here we present new statistical analyses of Q-scores based on its application to ∼ 10,000 maps and models archived in EMDB and PDB. Further we introduce two new metrics based on Q-score : Q-relative-all and Q-relative-resolution to compare a map and model to all entr

SUBMITTER: Pintilie G 

PROVIDER: S-EPMC11760781 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature