Methylene blue at recommended concentrations alters metabolism in early zebrafish development.

ABSTRACT: Methylene blue (MB) is an antifungal agent widely used during critical stages of zebrafish development. Most guidelines recommend 0.00005% or 0.0001% of MB for embryo/larval rearing. The Organisation for Economic Co-operation and Development zebrafish embryo toxicity test guideline omits MB recommendations, leading to inconsistent MB use in zebrafish research. Because MB affects oxidative energy metabolism in vitro and in vivo, we investigate possible metabolic effects of recommended MB concentrations in developing zebrafish (1-5 days post-fertilization (dpf)). MB increases O₂ consumption rate at 1 dpf, followed by an overall reduction in oxidative energy metabolism in post-hatch eleutheroembryos (4-5 dpf). Concomitantly, mitochondrial transcripts decrease in 1 and 4 dpf zebrafi

SUBMITTER: Nipu N 

PROVIDER: S-EPMC11760885 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature