Knockout of <i>dhx38</i> Causes Inner Ear Developmental Defects in Zebrafish.

ABSTRACT: Background: Alternative splicing is essential for the physiological and pathological development of the inner ear. Disruptions in this process can result in both syndromic and non-syndromic forms of hearing loss. DHX38, a DEAH box RNA helicase, is integral to pre-mRNA splicing regulation and plays critical roles in development, cell differentiation, and stem cell maintenance. However, its specific role in inner ear development remains undefined. Here, we utilized a dhx38 knockout zebrafish model to monitor the ear morphology and elucidate a crucial role for DHX38 in the development of the zebrafish inner ear. Methods: Bright-field morphological analysis and in situ hybridization were performed to observe ear morphology changes. Immunofluorescence and semi-quantitative

SUBMITTER: Ren M 

PROVIDER: S-EPMC11760894 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature