Proteomic profiling of zinc homeostasis mechanisms in <i>Pseudomonas aeruginosa</i> through data-dependent and data-independent acquisition mass spectrometry.

ABSTRACT: Zinc is central to the function of many proteins, yet the mechanisms of zinc homeostasis and their interplay with other cellular systems remain underexplored. In this study, we employ data-dependent acquisition (DDA) and data-independent acquisition (DIA) mass spectrometry to investigate proteome changes in Pseudomonas aeruginosa under conditions of different zinc availability. Using these methods, we detected 2143 unique proteins, 1578 of which were identified by both DDA and DIA. We demonstrated that most of the previously described Zn homeostasis systems exhibit proteomic responses that follow similar trends to those seen in transcriptomics studies. However, some proteins that are considered instrumental in Zn homeostasis, notably those in Zn transporter ZnuABC, were not detected

SUBMITTER: Meyer AC 

PROVIDER: S-EPMC11761036 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature