Enforced E-selectin ligand installation enhances homing and efficacy of adoptively transferred T cells.

ABSTRACT: Adoptive T-cell transfer has revolutionized the treatment of hematological malignancies. However, this approach has had very limited success in treating solid tumors, largely due to inadequate infiltration of vascularly administered T cells at tumor sites. The shear-resistant interaction between endothelial E-selectin and its cognate ligand expressed on leukocytes, sialyl Lewis X (sLe^X), is an essential prerequisite for extravasation of circulating leukocytes. Here, we report that enforced E-selectin ligand expression (enforced sLe^X display) on antigen-specific T cells can be achieved by fucosylating cells via cell surface treatment with the human α1-3-fucosyltransferase, FUT6 ("exofucosylation"), or via Golgi-targeted FUT6 overexpression ("Golgi-fucosylation"). Howev

SUBMITTER: Hou Y 

PROVIDER: S-EPMC11761414 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature