Targeted protein relocalization via protein transport coupling.

ABSTRACT: Subcellular protein localization regulates protein function and can be corrupted in cancers¹ and neurodegenerative diseases^2,3. The rewiring of localization to address disease-driving phenotypes would be an attractive targeted therapeutic approach. Molecules that harness the trafficking of a shuttle protein to control the subcellular localization of a target protein could enforce targeted protein relocalization and rewire the interactome. Here we identify a collection of shuttle proteins with potent ligands amenable to incorporation into targeted relocalization-activating molecules (TRAMs), and use these to relocalize endogenous proteins. Using a custom imaging analysis pipeline, we show that protein steady-state localization can be modulated through molecular couplin

SUBMITTER: Ng CSC 

PROVIDER: S-EPMC11761438 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature