The ratio of Wnt signaling activity to Sox2 transcription factor levels predicts neuromesodermal fate potential.

ABSTRACT: Neuromesodermal progenitors (NMPs) are a vertebrate cell type that contribute descendants to both the spinal cord and the mesoderm. The undifferentiated bipotential NMP state is maintained when both Wnt signaling is active and Sox2 is present. We used transgenic reporter lines to live-image both Wnt activity and Sox2 levels in NMPs and observed a unique cellular ratio in NMPs compared to NMP-derived mesoderm or neural tissue. We used this unique signature to identify the previously unknown anatomical position of a progenitor population that gives rise to the midline tissues of the floor plate of the spinal cord and the mesodermal notochord. Thus, quantification of the active Wnt signaling to Sox2 ratio can be used to predict and identify cells with neuromesodermal potential. We also develo

SUBMITTER: Morabito RD 

PROVIDER: S-EPMC11761523 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature