Physiologically Relevant Coculture Model for Oral Microbial-Host Interactions.

ABSTRACT: Understanding microbial-host interactions in the oral cavity is essential for elucidating oral disease pathogenesis and its systemic implications. In vitro bacteria-host cell coculture models have enabled fundamental studies to characterize bacterial infection and host responses in a reductionist yet reproducible manner. However, existing in vitro coculture models fail to replicate the physiological oxygen gradients critical for studying these interactions. Here, we present an asymmetric gas coculture system that simulates the oral microenvironment by maintaining distinct normoxic and anaerobic conditions for gingival epithelial cells and anaerobic bacteria, respectively. Using Fusobacterium nucleatum, a key oral pathobiont, we demonstrate that the system preserves bac

SUBMITTER: Pang Z 

PROVIDER: S-EPMC11761807 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature