Temporal transcriptional profiling of host cells infected by a veterinary alphaherpesvirus using nanopore sequencing.

ABSTRACT: In our research, we performed temporal transcriptomic profiling of host cells infected with Equid alphaherpesvirus 1 (EHV-1) by utilizing direct cDNA sequencing based on nanopore MinION technology. The sequencing reads were harnessed for transcript quantification at various time points. Viral infection-induced differential gene expression was identified through the edgeR package. The identified genes were segmented into six groups based on their kinetic characteristics. The initial three clusters encompass immediate-early response genes, typically transcription factors and elements of antiviral signaling pathways. These genes were either upregulated (cluster 1) or downregulated (clusters 2 and 3) during the early infection phase. The remaining three clusters include late response genes. In

SUBMITTER: Tombacz D 

PROVIDER: S-EPMC11762278 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature