Development and characterization of in vitro inducible immortalization of a murine microglia cell line for high throughput studies.

ABSTRACT: There are few in vitro models available to study microglial physiology in a homeostatic context. Recent approaches include the human induced pluripotent stem cell model, but these can be challenging for large-scale assays and may lead to batch variability. To advance our understanding of microglial biology while enabling scalability for high-throughput assays, we developed an inducible immortalized murine microglial cell line using a tetracycline expression system. The addition of doxycycline facilitates rapid cell proliferation, allowing for population expansion. Upon withdrawal of doxycycline, this monoclonal microglial cell line differentiates, resembling in vivo microglial physiology as demonstrated by the expression of microglial genes, innate immune responses, chemotaxis, and phagocy

SUBMITTER: Yeh H 

PROVIDER: S-EPMC11762310 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature