Berberine decorated zinc oxide loaded chitosan nanoparticles a potent anti cancer agent against breast cancer.

ABSTRACT: Breast cancer ranks as the second leading reason of cancer mortality among females globally, emphasizing the critical need for novel anticancer treatments. In current work, berberine-zinc oxide conjugated chitosan nanoparticles were synthesized and characterized using various characterization techniques. The cytotoxic effects of CS-ZnO-Ber NPs on MCF-7 cells were assessed using the MTT assay. Also, annexin V-FITC/PI double staining, Hoechst 33,342 staining, caspases-8 and 9 activity assays, and cell cycle analysis were performed. Furthermore, the mRNA levels of Bax and Bcl-2 genes were quantified using qPCR. Additionally, cell migration was evaluated using a scratch assay. The IC₅₀ value of NPs against MCF-7 cells measured 7.41 µg/mL. Apoptosis induction in NP-treated cells was

SUBMITTER: Esnaashari F 

PROVIDER: S-EPMC11762321 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature