Optimized Method to Generate Well-Characterized Macrophages from Induced Pluripotent Stem Cells.

ABSTRACT: Background/Objectives: Macrophages play a pivotal role in various pathogenic processes, necessitating the development of efficient differentiation techniques to meet the high demand for these cells in research and therapy. Human macrophages can be obtained via culturing peripheral blood monocytes; however, this source has limited yields and requires patient contact for each proposed use. In addition, it would be difficult to perform gene editing on peripheral blood monocytes. The objectives of this study are to define a robust and consistent method for the differentiation of induced pluripotent stem cells (iPSCs) into macrophages that can address these needs for recurrent studies with high yields and the potential for gene editing. Methods: We refined the traditional embryoid

SUBMITTER: Hai Q 

PROVIDER: S-EPMC11762477 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature