Investigating BoLA Class II DRB3*009:02 carrying cattle in Japan.

ABSTRACT: Enzootic bovine leukosis (EBL) is a malignant lymphoma of cattle that is mainly caused by bovine leukemia virus (BLV) infection. In this study, PCR-RFLP was used to investigate the frequency of the DRB3*009:02 allele in several farms with different herd management practices in Japan. A total of 742 Holsteins (384) and Japanese Blacks (230) were used as the sample size for the study, which was larger than the number of cattle in the study area with a confidence level of 95 % and a margin of error of 8. PBMCs isolated from whole blood from clinically healthy cattle were used for examination. The presence of BLV provirus infection was determined by qPCR targeting the env region. BLV antibodies were detected using a commercial ELISA kit. The results showed that 35 cattle were heterozygous for

SUBMITTER: Fujimori S 

PROVIDER: S-EPMC11762616 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature