Comparative Structural and Biophysical Investigation of <i>Lycosa erythrognatha</i> Toxin I (LyeTx I) and Its Analog LyeTx I-b.

ABSTRACT: Background/Objectives: This study investigates the structural and biophysical properties of the wild-type antimicrobial peptide LyeTx I, isolated from the venom of the spider Lycosa erythrognatha, and its analog LyeTx I-b, designed to enhance antibacterial activity, selectivity, and membrane interactions by the acetylation and increased amphipathicty. Methods: To understand the mechanisms behind these enhanced properties, comparative analyses of the structural, topological, biophysical, and thermodynamic aspects of the interactions between each peptide and phospholipid bilayers were evaluated. Both peptides were isotopically labeled with ²H₃-Ala and ¹⁵N-Leu to facilitate structural studies via NMR spectroscopy. Results: Circular

SUBMITTER: de Souza AN 

PROVIDER: S-EPMC11762800 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature