Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems.

ABSTRACT: This study evaluates the potential of using pond water eDNA to reflect the surrounding terrestrial plant communities, aiming to develop a sustainable, large-scale, and long-term monitoring method for plant diversity in forest ecosystems. Water samples were collected four times from two ponds with different vegetation types during the late spring to autumn seasons in Japan. eDNA was extracted from dissolved particles fractionated by sequential filtration through pore sizes of 200 µm, 5 µm, and 0.45 µm, followed by high-throughput amplicon sequencing targeting the plant rbcL gene. By comparing field surveys with the eDNA data, we identified 79% and 63% of plant families and genera, respectively, suggesting that pond water eDNA may reflect the surrounding terrestrial plant ecosystem. Addition

SUBMITTER: Bozdogan D 

PROVIDER: S-EPMC11762844 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature