<i>Ex-vivo</i> investigation of human salivary microbial growth with lysogeny broth for translational research-A pilot study.

ABSTRACT:

Background/purpose

Salivary microbiome has become a surrogate indicator of oral disease due to its collective reservoirs and convenience in sampling. However, failed clinical trials often lead to wastes of resources, indicating a need for preclinical models. In this pilot study, we aimed to compare the salivary microbiome by metagenomics analysis before and after lysogeny broth culture for prospective translational studies.

Materials and methods

The study cohort included seven patients with severe periodontitis (Stage III/IV, Grade C), from whom unstimulated saliva was collected. The salivary microbiome was sequenced over the 16S rRNA gene V3-V4 hypervariable regions at baseline and after 6 hours of lysogeny broth culture.

Results

The results revealed changes in saliv

SUBMITTER: Yang YH 

PROVIDER: S-EPMC11762923 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature