MicroRNA-21 as a Regulator of Cancer Stem Cell Properties in Oral Cancer.

ABSTRACT: Oral squamous cell carcinoma (OSCC) is a highly aggressive malignancy with poor prognosis, mainly due to the presence of cancer stem cells (CSCs), a small subpopulation of cells that contribute to therapy resistance and tumor progression. The principal objective of this study was to investigate the role of miRNA-21 in the maintenance of cancer cell stemness and the possibility of altering it. The CD44 antigen was used as a marker for CSC isolation from oral cancer cell cultures. CD44+ and CD44- populations were sorted via magnetic separation. miRNA-21 inhibition was performed in CD44+ cells via transfection. CD44+ cells possessed a significantly higher migration and invasion potential compared to CD44- cells, higher levels of miRNA-21 (p = 0.004) and β-catenin (p = 0.005), an

SUBMITTER: Jaksic Karisik M 

PROVIDER: S-EPMC11763652 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature