Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay.

ABSTRACT: Lateral flow immunoassays (LFIAs) are widely used for their low cost, simplicity, and rapid results; however, enhancing their reliability requires the meticulous selection of ligands and nanoparticles (NPs). SiO₂@QD@SiO₂ (QD²) nanoparticles, which consist of quantum dots (QDs) embedded in a silica (SiO₂) core and surrounded by an outer SiO₂ shell, exhibit significantly higher fluorescence intensity (FI) compared to single QDs. In this study, we prepared QD²@PEG@Aptamer, an aptamer conjugated with QD² using succinimidyl-[(N-maleimidopropionamido)-hexaethyleneglycol]ester, which is 130 times brighter than single QDs, for detecting carbohydrate antigen (CA) 19-9 through LFIA. For LFIA optimization, we determined the

SUBMITTER: Yoo K 

PROVIDER: S-EPMC11763673 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature