γδ T Are Significantly Impacted by CLL Burden but Only Mildly Influenced by M-MDSCs.

ABSTRACT:

Background/objectives

The current study explores the impact of CLL on γδ T cells and, in an attempt to better understand the sources of immunosuppression, assesses the impact of M-MDSCs on γδ T cells in vitro.

Methods

The study included 163 CLL patients and 34 healthy volunteers. γδ T cells were screened with flow cytometry, including NKG2D, Fas, FasL, and TRAIL staining. Additionally, to deepen understanding of the immunosuppressive impact of CLL on γδ T, a set of in vitro co-cultures of γδ T and M-MDSCs was performed.

Results

RNAseq revealed significant, though relatively minor, changes in the transcriptome. Functional analyses showed a minor drop in cytotoxic potential against CLL cells. Finally, depletion of M-MDSCs from CLL-derived peripheral blood mononuclear ce

SUBMITTER: Zarobkiewicz M 

PROVIDER: S-EPMC11763719 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature