In Vitro Evaluation of the Safety and Efficacy of <i>Cibisatamab</i> Using Adult Stem Cell-Derived Organoids and Colorectal Cancer Spheroids.

ABSTRACT: Objectives: Developing ex vivo models that replicate immune-tumor interactions with high fidelity is essential for advancing immunotherapy research, as traditional two-dimensional in vitro systems often lack the complexity required to fully represent these interactions. Methods: In this study, we establish a comprehensive 3D redirect lysis (3D-RDL) assay using colorectal cancer spheroids and adult stem cell-derived, healthy human organoids to evaluate the efficacy and safety profile of Cibisatamab, a bispecific antibody targeting carcinoembryonic antigens (CEAs) on cancer cells and CD3 on T cells. This model allows us to assess cytotoxic activity and immune responses, capturing variations in therapeutic response not observable in simpler systems. Our model integrates l

SUBMITTER: Anstett V 

PROVIDER: S-EPMC11763756 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature