The Pre-Polarization and Concentration of Cells near Micro-Electrodes Using AC Electric Fields Enhances the Electrical Cell Lysis in a Sessile Drop.

ABSTRACT: Cell lysis is the starting step of many biomedical assays. Electric field-based cell lysis is widely used in many applications, including point-of-care (POC) applications, because it provides an easy one-step solution. Many electric field-based lysis methods utilize micro-electrodes to apply short electric pulses across cells. Unfortunately, these cell lysis devices produce relatively low cell lysis efficiency as electric fields do not reach a significant portion of cells in the sample. Additionally, the utility of syringe pumps for flow cells in and out of the microfluidics channel causes cell loss and low throughput cell lysis. To address these critical issues, we suspended the cells in a sessile drop and concentrated on the electrodes. We used low-frequency AC electric fields (1 Vpp, 0-

SUBMITTER: Kaphle K 

PROVIDER: S-EPMC11763957 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature