Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions.

ABSTRACT: Since lead can cause severe effects on living organisms' health and life, the regular monitoring of Pb levels in water and soil is of particular significance. Recently, it was shown that lead ions can also be detected using affinity-based biosensors, namely, using aptamers as recognition elements. In most cases, thrombin binding aptamer (TBA) was utilized; however, there are more examples of DNA aptamers which could also serve that purpose. Herein, we present studies on the electrochemical detection of lead ions using PS2M aptamer, which contains several guanine nucleotides, as the receptor element. Firstly, the method of aptamer-based layer fabrication was optimized along with the choice of a redox active indicator, which was a source of current signal. The experiments revealed the possib

SUBMITTER: Zaras I 

PROVIDER: S-EPMC11764081 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature