Fluorescence Lifetime Imaging of NAD(P)H in Patients' Lymphocytes: Evaluation of Efficacy of Immunotherapy.

ABSTRACT:

Background

The wide variability in clinical responses to anti-tumor immunotherapy drives the search for personalized strategies. One of the promising approaches is drug screening using patient-derived models composed of tumor and immune cells. In this regard, the selection of an appropriate in vitro model and the choice of cellular response assay are critical for reliable predictions. Fluorescence lifetime imaging microscopy (FLIM) is a powerful, non-destructive tool that enables direct monitoring of cellular metabolism on a label-free basis with a potential to resolve metabolic rearrangements in immune cells associated with their reactivity.

Objective

The aim of the study was to develop a patient-derived glioma explant model enriched by autologous peripheral lymphocytes and

SUBMITTER: Yuzhakova DV 

PROVIDER: S-EPMC11764258 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature