Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens.

ABSTRACT: Background/Objectives: Performing hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) on the same specimen slide provides advantages that include specimen conservation and the ability to combine the H&E context with biomarker expression at the individual cell level. We previously used invisible deposited chromogens and dual-camera imaging, including monochrome and color cameras, to implement simultaneous H&E and IHC. Using this approach, conventional H&E staining could be simultaneously viewed in color on a computer monitor alongside a monochrome video of the invisible IHC staining, while manually scanning the specimen. Methods: We have now simplified the microscope system to a single camera and increased the IHC multiplexing to four biomarkers using translati

SUBMITTER: Morrison LE 

PROVIDER: S-EPMC11764411 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature