Hybridization Design and High-Throughput Screening of Peptides with Immunomodulatory and Antioxidant Activities.

ABSTRACT: With the increasing recognition of the role of immunomodulation and oxidative stress in various diseases, designing peptides with both immunomodulatory and antioxidant activities has emerged as a promising therapeutic strategy. In this study, a hybridization design was applied as a powerful method to obtain multifunctional peptides. A total of 40 peptides with potential immunomodulatory and antioxidant activities were designed and screened. First, molecular docking was employed to screen peptides with a high binding affinity to MD2, a key receptor protein in the NFκB immune pathway. For the in vitro high-throughput screening, we constructed a reporter gene-based stable cell line, IPEC-J2-Lucia ARE cells, which was subsequently used to screen peptides with antioxidant activity. Furthermore,

SUBMITTER: Wang J 

PROVIDER: S-EPMC11764585 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature