Various Options for Covalent Immobilization of Cysteine Proteases-Ficin, Papain, Bromelain.

ABSTRACT: This study explores various methods for the covalent immobilization of cysteine proteases (ficin, papain, and bromelain). Covalent immobilization involves the formation of covalent bonds between the enzyme and a carrier or between enzyme molecules themselves without a carrier using a crosslinking agent. This process enhances the stability of the enzyme and allows for the creation of preparations with specific and controlled properties. The objective of this study is to evaluate the impact of covalent immobilization under different conditions on the proteolytic activity of the enzymes. The most favorable results were achieved by immobilizing ficin and bromelain through covalent bonding to medium and high molecular weight chitosans, using 5 and 3.33% glutaraldehyde solutions, respectively. F

SUBMITTER: Holyavka MG 

PROVIDER: S-EPMC11764635 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature