Electroacupuncture enhances the mitophagy of granulosa cells in premature ovarian insufficiency model mice by inactivating the hippo-yes-associated protein/transcriptional co-activator with postsynaptic density protein, drosophila disc large tumor suppressor, and zonula occludens-1 protein binding motif pathway.

ABSTRACT:

Objective

To investigate the potential mechanism of electroacupuncture (EA) in alleviating premature ovarian insufficiency (POI) and to provide a theoretical basis for EA treatment of POI.

Methods

For this purpose, a POI mice model was developed by injecting 12 mg/kg busulfan and 120 mg/kg cyclophosphamide intraperitoneally to induce POI. It was then proceeded by EA intervention at Guanyuan (CV4) acupoint on the second day following modeling. Similarly, apoptosis in ovarian granulosa cells was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling staining, while enzyme-linked immunosorbent assay was employed for measuring serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E₂), and anti-müllerian hormone (AMH) levels. M

SUBMITTER: Jiaman WU 

PROVIDER: S-EPMC11764945 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature