A Comparative Transcriptome and Proteome Analysis of the Molecular Mechanism Underlying Anterior to Dorsal Eye Rotation in the Celestial-Eye Goldfish (<i>Carassius auratus</i>).

ABSTRACT: Goldfish (Carassius auratus), subjected to millennia of artificial selection and breeding, have diversified into numerous ornamental varieties, such as the celestial-eye (CE) goldfish, noted for its unique dorsal eye rotation. Previous studies have primarily focused on anatomical modifications in CE goldfish eyes, yet the molecular underpinnings of their distinctive eye orientation remain poorly understood. This study employed high-throughput transcriptome and proteome sequencing on 110-day-old full-sibling CE goldfish, which displayed either anterior or upward eye rotations. Verification of these findings was conducted using quantitative PCR (qPCR) for transcriptomic data and parallel reaction monitoring (PRM) for proteomic analysis. Our research identified 73,685 genes and 7717 pr

SUBMITTER: Li R 

PROVIDER: S-EPMC11765313 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature