Whole Genome Sequencing and Comparative Genomics Analysis of Goat-Derived <i>Klebsiella oxytoca</i>.

ABSTRACT: Background: This research aims to enhance the genomic database of Klebsiella oxytoca by identifying virulence genes through the whole genome sequencing and comparative analysis of a goat-derived K. oxytoca (KOHN1) strain, while clarifying the relationship between its genetic evolution and virulence, ultimately providing a theoretical foundation for clinical prevention and diagnosis. Methods: Third-generation Oxford Nanopore Technologies (ONT) sequencing and second-generation Illumina sequencing were used to sequence the strain and analyze the database annotations. Screening for 10 virulence genes was conducted using PCR. Comparative genomic analyses of the strain KOHN1 with four human-derived K. oxytoca model strains were performed using collinearity anal

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11765384 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature