Genome-Wide microRNA Expression Profiling in Human Spermatozoa and Its Relation to Sperm Quality.

ABSTRACT:

Background

Sperm samples are separated into bad and good quality samples in function of their phenotype, but this does not indicate their genetic quality.

Methods

Here, we used GeneChip miRNA arrays to analyze microRNA expression in ten semen samples selected based on high-magnification morphology (score 6 vs. score 0) to identify miRNAs linked to sperm phenotype.

Results

We found 86 upregulated and 21 downregulated miRNAs in good-quality sperm (score 6) compared with bad-quality sperm samples (score 0) (fold change > 2 and p-value < 0.05). MiR-34 (FC × 30, p = 8.43 × 10^-8), miR-30 (FC × 12, p = 3.75 × 10^-6), miR-122 (FC × 8, p = 0.0031), miR-20 (FC × 5.6, p = 0.0223), miR-182

SUBMITTER: Cassuto NG 

PROVIDER: S-EPMC11765444 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature