Cell-to-Cell Natural Transformation Mediated Efficient Plasmid Transfer Between <i>Bacillus</i> Species.

ABSTRACT: Horizontal gene transfer (HGT) plays a pivotal role in bacterial evolution, shaping the genetic diversity of bacterial populations. It can occur through mechanisms such as conjugation, transduction, and natural transformation. Bacillus subtilis, a model Gram-positive bacterium, serves not only as a robust system for studying HGT but also as a versatile organism with established industrial applications, such as producing industrial enzymes, antibiotics, and essential metabolites. In this study, we characterize a novel method of plasmid transfer, termed Cell-to-Cell Natural Transformation for Plasmid Transfer (CTCNT-P), which efficiently facilitates plasmid transfer between naturally competent B. subtilis strains. This method involves co-culturing donor and recipient cells unde

SUBMITTER: Wang C 

PROVIDER: S-EPMC11765539 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature