Batch-to-Batch Variation and Patient Heterogeneity in Thymoglobulin Binding and Specificity: One Size Does Not Fit All.

ABSTRACT: Background: Thymoglobulin is used to prevent allograft rejection and is being explored at low doses as intervention immunotherapy in type 1 diabetes. Thymoglobulin consists of a diverse pool of rabbit antibodies directed against many different targets on human thymocytes that can also be expressed by other leukocytes. Since Thymoglobulin is generated by injecting rabbits with human thymocytes, this conceivably leads to differences between Thymoglobulin batches. Methods: We compared different batches for antibody composition and variation between individuals in binding to PBMC and T cell subsets, and induction of cytokines. Four different batches of Thymoglobulin were directly conjugated with Alexa-Fluor 647. Blood was collected from five healthy donors, and PBMCs were isolate

SUBMITTER: den Hollander NHM 

PROVIDER: S-EPMC11765605 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature