Molecular Mechanism During Mycelium Subculture Degeneration of <i>Volvariella volvacea</i>.

ABSTRACT: Periodic mycelial subculture is a method commonly used for the storage of edible mushrooms, but excessive subculturing can lead to the degeneration of strains. In this study, the Volvariella volvacea strain V971(M0) was successively subcultured on PDA medium every 4 days, and one generation of strains was preserved every 4 months. Thus, five generations of subcultured strains (M1-M5) were obtained after 20 months of mycelial subculturing, their production traits were determined, and transcriptomic analysis was performed using RNA-seq; the differentially expressed genes were verified via RT-qPCR. The results showed that as the number of subcultures increased, the diameter of the mycelium and biological efficiency gradually decreased; in addition, the time in which the primordium form

SUBMITTER: Feng L 

PROVIDER: S-EPMC11766388 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature