Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 Diabetes.

ABSTRACT: Type 1 diabetes (T1D) is related to the autoimmune destruction of β-cells, leading to their almost complete absence in patients with longstanding T1D. However, endogenous insulin secretion persists in such patients as evidenced by the measurement of plasma C-peptide. Recently, a low level of insulin has been found in non-β islet cells of patients with longstanding T1D, indicating that other islet cell types may contribute to persistent insulin secretion. The present study aimed to test the ability of various antibodies to detect insulin in insulin-deficient islets of T1D patients. Pancreatic autopsies from two children with recent-onset T1D, two adults with longstanding T1D, and three control subjects were examined using double immunofluorescent labeling with antibodies to insulin, glucago

SUBMITTER: Krivova Y 

PROVIDER: S-EPMC11766825 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature