Azole Resistance and ERG11 Mutation in Clinical Isolates of Candida tropicalis.

ABSTRACT: We investigated the molecular mechanisms underlying azole resistance in seven Candida tropicalis isolates that caused candidemia and candiduria in Paraná, Brazil (2016-2022). Biofilm production, antifungal susceptibility testing, multilocus sequence typing, amplification and sequencing of ERG11, and quantification of ERG11, MDR1, and CDR1 expression levels were performed. Notably, five isolates (71.4%) were from urine samples and two (28.6%) were from blood samples. All strains were biofilm producers, with levels ranging from moderate to strong. The minimum inhibitory concentration (MIC) values ranged from 8->64 mg/L for fluconazole and 0.25-1 mg/L for voriconazole. All isolates had mutations in ERG11; Y132F and Y257N were predominant (71.4%), foll

SUBMITTER: Siqueira AC 

PROVIDER: S-EPMC11767116 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature