Light Regulates Secreted Metabolite Production and Antagonistic Activity in <i>Trichoderma</i>.

ABSTRACT: Secondary metabolism is one of the main mechanisms Trichoderma uses to explore and colonize new niches, and 6-pentyl-α-pyrone (6-PP) is an important secondary metabolite in this process. This work focused on standardizing a method to investigate the production of 6-PP. Ethanol and ethyl acetate were both effective solvents for quantifying 6-PP in solution and had limited solubility in potato-dextrose-broth media. The 6-PP extraction using ethyl acetate provided a rapid and efficient process to recover this metabolite. The 6-PP was readily produced during the development of Trichoderma atroviride growing in the dark, but light suppressed its production. The 6-PP was purified, and its spectrum by nuclear magnetic resonance and mass spectroscopy was identical to that of commerci

SUBMITTER: Esquivel-Naranjo EU 

PROVIDER: S-EPMC11767173 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature