Targeting Transcriptional Regulators Affecting Acarbose Biosynthesis in <i>Actinoplanes</i> sp. SE50/110 Using CRISPRi Silencing.

ABSTRACT: Acarbose, a pseudo-tetrasaccharide produced by Actinoplanes sp. SE50/110, is an α-glucosidase inhibitor and is used as a medication to treat type 2 diabetes. While the biosynthesis of acarbose has been elucidated, little is known about its regulation. Gene silencing using CRISPRi allows for the identification of potential regulators influencing acarbose formation. For this purpose, two types of CRISPRi vectors were established for application in Actinoplanes sp. SE50/110. The pCRISPomyces2i vector allows for reversible silencing, while the integrative pSETT4i vector provides a rapid screening approach for many targets due to its shorter conjugation time into Actinoplanes sp. These vectors were validated by silencing the known acarbose biosynthesis genes acbB and

SUBMITTER: Dymek S 

PROVIDER: S-EPMC11767292 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature