Recombinant Production of Bovine α_S1-Casein in Genome-Reduced <i>Bacillus subtilis</i> Strain IIG-Bs-20-5-1.

ABSTRACT:

Background

Cow's milk represents an important protein source. Here, especially casein proteins are important components, which might be a promising source of alternative protein production by microbial expression systems. Nevertheless, caseins are difficult-to-produce proteins, making heterologous production challenging. However, the potential of genome-reduced Bacillus subtilis was applied for the recombinant production of bovine α_S1-casein protein.

Methods

A plasmid-based gene expression system was established in B. subtilis allowing the production of his-tagged codon-optimized bovine α_S1-casein. Upscaling in a fed-batch bioreactor system for high cell-density fermentation processes allowed for efficient recombinant α_S1-casein

SUBMITTER: Biermann L 

PROVIDER: S-EPMC11767299 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature