Screening of Solvent Systems for Countercurrent Chromatography Separation of Polar Constituents from <i>Ginkgo biloba</i> L. Seeds.

ABSTRACT: The separation of large polar constituents presents a substantial challenge in natural product research when employing column chromatography techniques, as the process is both complex and time-consuming. In this study, an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride solvent system was developed and utilized for the countercurrent chromatography of polar constituents from Ginkgo biloba L. seeds. Five polar constituents were effectively isolated using an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride (2:2:0.8:3, v/v) solvent system using a two-step countercurrent chromatography method. In the initial countercurrent chromatography process, three constituents were successf

SUBMITTER: Hu R 

PROVIDER: S-EPMC11767392 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature