In Vitro Model of Vascular Remodeling Under Microfluidic Perfusion.

ABSTRACT: We developed a portable microfluidic system that combines spontaneous lumen formation from human umbilical endothelial cells (HUVECs) in fibrin-collagen hydrogels with active perfusion controlled by a braille actuator. Adaptive interstitial flow and feedthrough perfusion switching enabled the successful culture of spontaneously formed naturally branched lumens for more than one month. We obtained many large-area (2 mm × 3 mm) long-term (more than 30 days per run) time-lapse image datasets of the in vitro luminal network using this microfluidic system. We also developed an automatic image analysis pipeline to extract the morphology of the lumen network and node-edge network structure weighted with segmentwise flow parameters. The automatic lumen area measurements revealed that almost all lu

SUBMITTER: Nishikata K 

PROVIDER: S-EPMC11767722 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature