Transcriptome Analysis of <i>Stephania yunnanensis</i> and Functional Validation of CYP80s Involved in Benzylisoquinoline Alkaloid Biosynthesis.

ABSTRACT: The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloid (BIA), with aporphine being the representative and most abundant compound, but our understanding of the biosynthesis of BIAs in this plant has been relatively limited. Previous research reported the genome of S. yunnanensis and preliminarily identified the norcoclaurine synthase (NCS), which is involved in the early stages of the BIA biosynthetic pathways. However, the key genes promoting the formation of the aporphine skeleton have not yet been reported. In this study, based on the differences in the content of crebanine and several other BIAs in different tissues, we conducted transcriptome sequencing of roots, stems, and leaves. We then identified candidate genes

SUBMITTER: Shi W 

PROVIDER: S-EPMC11767795 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature