Direct Hot Solid-Liquid Extraction (DH-SLE): A High-Yield Greener Technique for Lipid Recovery from Coffee Beans.

ABSTRACT: Soxhlet extraction is a method recommended by the Association of Official Analytical Chemists (AOAC) to determine the lipid content in plant samples. Generally, n-hexane (toxicity grade 5) is used as the solvent (≈300 mL; ≈30 g sample) at boiling temperatures (69 °C) for long times (≤16 h) under a chilled water reflux (≈90 L/h), proportionally aggravated by the number of repetitions and samples determined. In this sense, the technique is neither safe nor sustainable for the analyst or the environment. This article presents the development of an alternative and more sustainable procedure for determining the lipid content in raw Arabica coffee beans. A 3³ full factorial design was used to perform direct hot solid-liquid extractions in 4 mL vials, varying the ground grains and solv

SUBMITTER: de Faria DC 

PROVIDER: S-EPMC11768105 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature