Discovery of Novel Diagnostic Biomarkers for Common Pathogenic <i>Nocardia</i> Through Pan-Genome and Comparative Genome Analysis, with Preliminary Validation.

ABSTRACT: The aim of this study was to reveal diagnostic biomarkers of considerable importance for common pathogenic Nocardia, utilizing pan-genomic and comparative genome analysis to accurately characterize clinical Nocardia infections. In this study, complete or assembled genome sequences of common pathogenic Nocardia and closely related species were obtained from NCBI as discovery and validation sets, respectively. Genome annotation was performed using Prokka software, and pan-genomic analysis and extraction of Nocardia core genes were performed using BPGA software. Comparative genome analysis of these core genes with the validation-set gene sequences was then performed using BLAT, with a threshold of 30% amino acid coverage and identity, in order to distinguish specif

SUBMITTER: Wang C 

PROVIDER: S-EPMC11768141 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature