Spermine Significantly Increases the Transfection Efficiency of Cationic Polymeric Gene Vectors.

ABSTRACT: Background/Objectives: Non-viral vectors have gained recognition for their ability to enhance the safety of gene delivery processes. Among these, polyethyleneimine (PEI) stands out as the most widely utilized cationic polymer due to its accessibility. Traditional methods of modifying PEI, such as ligand conjugation, chemical derivatization, and cross-linking, are associated with intricate preparation procedures, limited transfection efficiency, and suboptimal biocompatibility. Methods: In this investigation, enhanced transfection efficiency was achieved through the straightforward physical blending of PEI carriers with spermine. Results: Transfection assays explored the maximal enhancement potential conferred by spermine, alongside further methodological refinements ai

SUBMITTER: Lv Y 

PROVIDER: S-EPMC11768368 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature